Camila Monteiro Siqueira1, Beatriz Costa1, Ana Maria Amorim2, Marta Gonçalves2, Venício Féo da Veiga2, Morgana Castelo-Branco3, Christina Takyia3, Patrícia Zancan4, Fernando Portela Câmara2, José Nelson Couceiro2, Carla Holandino1

1 Departamento de Medicamentos-Faculdade de Farmácia, Universidade Federal do Rio de Janeiro – UFRJ, Rio de Janeiro, Brazil
2 Instituto de Microbiologia Professor Paulo de Góes, Universidade Federal do Rio de Janeiro – UFRJ, Rio de Janeiro, Brazil
3 Instituto de Ciências Biomédicas, Universidade Federal do Rio de Janeiro – UFRJ, Rio de, Janeiro, Brazil
4 Departamento de Fármacos-Faculdade de Farmácia, Universidade Federal do Rio de Janeiro – UFRJ, Rio de Janeiro, Brazil 

O bioterápico denominado Influenzinum RC, foi preparado através da ultradiluição (diluição homeopática 30dH) do vírus Influenza A (A/Aichi/2/68 H3N20). As alterações celulares induzidas por este bioterápico foram analisadas através de  analises bioquímicas e microscopia óptica e eletrônica.

O Influenzinum RC não causou efeito citotóxico nem alterações morfológicas em célula MDCK (Madin–Darby canine kidney) Apos 30 dias foi observado um aumento na taxa de mitose. A atividade mitocondrial foi alterada após tratamento  com 10 e 30 dias. Ocorreu diminuição de PFK-1 e em células  de macrófagos J774.48 tratadas   a  citocina TNF-α aumentou  nos sobrenadantes das culturas de macrófagos.

H3N2 homeopathic influenza virus solution modifies cellular and biochemical aspects of MDCK and J774G8 cell lines

Objectives

To develop a biotherapy prepared from the infectious influenza A virus (A/Aichi/2/68 H3N2) and to verify its in vitro response.

Methods

The ultradiluted influenza virus solution was prepared in the homeopathic dilution 30dH, it was termed Influenzinum RC. The cellular alterations induced by this preparation were analyzed by optical and electron microscopy, MTT and neutral red assays. Glycolytic metabolism (PFK-1) was studied by spectrophotometric assay. Additionally, the production of tumor necrosis factor-α (TNF-α) by J774.G8 macrophage cells was quantified by ELISA before and after infection with H3N2 influenza virus and treatment

Results

Influenzinum RC did not cause cytotoxic effects but induced morphological alterations in Madin–Darby canine kidney (MDCK) cells. After 30 days, a significant increase (p < 0.05) in mitosis rate was detected compared to control. MDCK mitochondrial activity was changed after treatment for 10 and 30 days. Treatment significantly diminished (p < 0.05) PFK-1 activity. TNF-α in biotherapy-stimulated J774.G8 macrophages indicated a significant (p < 0.05) increase in this cytokine when the cell supernatant was analyzed.

Conclusion

Referencia: Homeopathy 2013; 102(1);31-40